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| Vargas Júnior,Sergio Farias; Costa,Vitória Gasperin Guazzelli; Oliveira,Fernando Caetano de; Goularte,Karina Lemos; Haas,Cristina Sangoi; Gasperin,Bernardo Garziera; Lucia Júnior,Thomaz. |
| Abstract: The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infertility; Insemination; Intrafollicular artificial insemination; Spermatozoa. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000700866 |
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| Costa,Vitória Gasperin Guazzelli; Vieira,Arnaldo Diniz; Schneider,Augusto; Rovani,Monique Tomazele; Gonçalves,Paulo Bayard Dias; Gasperin,Bernardo Garziera. |
| ABSTRACT: Previous studies have evaluated the effects of different reproductive procedures on discomfort markers in sheep and cattle. Such studies may help stimulate the adoption of techniques that are more beneficial for animal welfare. However, markers that are commonly used to evaluate discomfort are highly influenced by external factors. To overcome this, several systemic markers can be evaluated to more precisely identify stress, pain, and inflammation. Such markers include cortisol, acute phase proteins, bradykinin, and substance P. We aimed to review the potential markers of stress, pain, and inflammation, and discuss how and when they are regulated after different stimuli related to reproductive procedures in cattle and sheep. Furthermore, we... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute phase proteins; Cortisol; Discomfort; Reproductive techniques. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200651 |
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